Called nucleotide
Set base to its complement
Flow index (0-based)
Signal intensity, normalized to homopolymer lengths
round(intensity * Multiplier) where Multiplier is 100.0 for FZ tags, and 256.0 for ZM tags. More efficient, because this is how intensities are stored in FZ/ZM tag.
Called homopolymer length
Zero-based position of the first nucleotide in the run, relative to start of the read. Takes strandness into account.
Flow call associated with a read