1 /* 2 This file is part of BioD. 3 Copyright (C) 2012-2013 Artem Tarasov <lomereiter@gmail.com> 4 5 Permission is hereby granted, free of charge, to any person obtaining a 6 copy of this software and associated documentation files (the "Software"), 7 to deal in the Software without restriction, including without limitation 8 the rights to use, copy, modify, merge, publish, distribute, sublicense, 9 and/or sell copies of the Software, and to permit persons to whom the 10 Software is furnished to do so, subject to the following conditions: 11 12 The above copyright notice and this permission notice shall be included in 13 all copies or substantial portions of the Software. 14 15 THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR 16 IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, 17 FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE 18 AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER 19 LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING 20 FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER 21 DEALINGS IN THE SOFTWARE. 22 23 */ 24 module bio.std.hts.sam.reader; 25 // 26 import bio.std.hts.bam.abstractreader; 27 import bio.std.hts.sam.header; 28 import bio.std.hts.bam.read; 29 import bio.std.hts.bam.reference; 30 import bio.std.hts.bam.referenceinfo; 31 import bio.core.utils.outbuffer; 32 import bio.core.utils.range; 33 34 import bio.core.utils.bylinefast; 35 alias ByLineFast _LineRange; 36 37 version(DigitalMars) { 38 import bio.std.hts.sam.utils.recordparser; 39 } else { 40 import bio.std.hts.sam.utils.fastrecordparser; 41 } 42 43 import std.stdio; 44 import std.array; 45 import std..string; 46 import std.range; 47 import std.algorithm; 48 import std.typecons; 49 import std.parallelism; 50 import std.process; 51 import std.exception; 52 import core.stdc..string; 53 54 BamRead _parseSamRecord(Tuple!(char[], SamReader, OutBuffer) t) { 55 auto r = parseAlignmentLine(cast(string)t[0], t[1]._header, t[2]); 56 BamRead result; 57 if (t[1]._seqprocmode) { 58 result = r; 59 } else { 60 auto storage = uninitializedArray!(ubyte[])(r.raw_data.length); 61 storage[] = r.raw_data[]; 62 result.raw_data = storage; 63 } 64 result.associateWithReader(t[1]); 65 return result; 66 } 67 68 private { 69 extern(C) size_t lseek(int, size_t, int); 70 bool isSeekable(ref File file) { 71 return lseek(file.fileno(), 0, 0) != ~0; 72 } 73 } 74 75 /// 76 class SamReader : IBamSamReader { 77 78 private { 79 version(gzippedSamSupport) { 80 void checkGunzip() { 81 auto gunzip = executeShell("gunzip -V"); 82 if (gunzip.status != 0) 83 throw new Exception("gunzip is not installed on this system, can't read gzipped SAM"); 84 } 85 86 File openSamFile(string filename) { 87 if (filename.length < 4) 88 throw new Exception("invalid name for SAM file: " ~ filename); 89 if (filename[$ - 3 .. $] == ".gz") { 90 checkGunzip(); 91 auto pipe = pipeShell("gunzip -c " ~ filename); 92 return pipe.stdout; 93 } else if (filename[$ - 4 .. $] == ".bam") { 94 throw new Exception("SAM reader can't read BAM file " ~ filename); 95 } else { 96 return File(filename); 97 } 98 } 99 100 } else { 101 102 File openSamFile(string filename) { 103 if (filename[$ - 4 .. $] == ".bam") { 104 throw new Exception("SAM reader can't read BAM file " ~ filename); 105 } else { 106 return File(filename); 107 } 108 } 109 110 } 111 } 112 113 /// 114 this(string filename) { 115 _file = openSamFile(filename); 116 _filename = filename; 117 _seekable = _file.isSeekable(); 118 _initializeStream(); 119 } 120 121 /// 122 bio.std.hts.sam.header.SamHeader header() @property { 123 return _header; 124 } 125 126 /// 127 const(bio.std.hts.bam.referenceinfo.ReferenceSequenceInfo)[] reference_sequences() @property const { 128 return _reference_sequences; 129 } 130 131 /// 132 bool hasReference(string reference) { 133 return null != (reference in _reference_sequence_dict); 134 } 135 136 /// 137 bio.std.hts.bam.reference.ReferenceSequence opIndex(string ref_name) { 138 enforce(hasReference(ref_name), "Reference with name " ~ ref_name ~ " is not present in the header"); 139 auto ref_id = _reference_sequence_dict[ref_name]; 140 return ReferenceSequence(null, ref_id, _reference_sequences[ref_id]); 141 } 142 143 /// Reads in SAM file. 144 auto reads() @property { 145 146 _LineRange lines = _lines; 147 if (_seekable) { 148 if (_filename !is null) { 149 auto file = openSamFile(_filename); 150 lines = ByLineFast(file); 151 } else { 152 _file.seek(0); 153 lines = ByLineFast(_file); 154 } 155 auto dummy = lines.front; 156 for (int i = 0; i < _lines_to_skip; i++) 157 lines.popFront(); 158 } 159 160 auto b = new OutBuffer(262144); 161 return lines.zip(repeat(this), repeat(b)).map!_parseSamRecord(); 162 } 163 164 /// 165 void assumeSequentialProcessing() { 166 _seqprocmode = true; 167 } 168 169 /// 170 std.range.InputRange!(bio.std.hts.bam.read.BamRead) allReads() @property { 171 return inputRangeObject(reads); 172 } 173 174 /// Filename 175 string filename() @property const { 176 return _filename; 177 } 178 private: 179 180 File _file; 181 bool _seekable; 182 string _filename; 183 _LineRange _lines; 184 ulong _lines_to_skip; 185 186 bool _seqprocmode; 187 188 SamHeader _header; 189 ReferenceSequenceInfo[] _reference_sequences; 190 int[string] _reference_sequence_dict; 191 192 void _initializeStream() { 193 auto header = Appender!(char[])(); 194 195 _lines = ByLineFast(_file); 196 197 while (!_lines.empty) { 198 auto line = _lines.front; 199 if (line.length > 0 && line[0] == '@') { 200 header.put(line); 201 header.put('\n'); 202 _lines_to_skip += 1; 203 _lines.popFront(); 204 } else { 205 break; 206 } 207 } 208 209 import core.memory; 210 GC.disable(); 211 _header = new SamHeader(cast(string)(header.data)); 212 GC.enable(); 213 214 _reference_sequences = new ReferenceSequenceInfo[_header.sequences.length]; 215 foreach (sq; _header.sequences) { 216 auto seq = ReferenceSequenceInfo(sq.name, sq.length); 217 auto n = cast(int)_reference_sequences.length; 218 _reference_sequence_dict[sq.name] = n; 219 _reference_sequences[_header.getSequenceIndex(seq.name)] = seq; 220 } 221 } 222 }