1 /*
2     This file is part of BioD.
3     Copyright (C) 2012    Artem Tarasov <lomereiter@gmail.com>
4 
5     Permission is hereby granted, free of charge, to any person obtaining a
6     copy of this software and associated documentation files (the "Software"),
7     to deal in the Software without restriction, including without limitation
8     the rights to use, copy, modify, merge, publish, distribute, sublicense,
9     and/or sell copies of the Software, and to permit persons to whom the
10     Software is furnished to do so, subject to the following conditions:
11     
12     The above copyright notice and this permission notice shall be included in
13     all copies or substantial portions of the Software.
14     
15     THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
16     IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
17     FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
18     AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
19     LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
20     FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER
21     DEALINGS IN THE SOFTWARE.
22 
23 */
24 module bio.std.hts.bam.baifile;
25 
26 public import bio.core.bgzf.chunk;
27 public import bio.std.hts.bam.bai.bin;
28 import bio.core.bgzf.virtualoffset;
29 import bio.std.hts.bam.constants;
30 
31 //TODO Remove stream dependency!
32 import contrib.undead.stream;
33 import std.system;
34 import std.exception;
35 import std.algorithm;
36 import std.conv;
37 import std.range;
38 import std.file;
39 import std.path;
40 
41 /// Represents index for a single reference
42 struct Index {
43     /// Information about bins
44     Bin[] bins;
45 
46     /// Virtual file offsets of first alignments overlapping 16384-byte windows
47     /// on the reference sequence. This linear index is used to reduce amount
48     /// of file seeks for region queries, since with its help one can reduce the
49     /// number of chunks to be investigated based on their end position.
50     ///
51     ///
52     /// Suppose you have a region [beg, end) and want to do a query.
53     ///
54     /// Here's the reference:
55     /// [....................!..............!.................................]
56     ///                     beg            end
57     ///
58     /// Here's the same reference with 16384-byte long windows:
59     /// [%...........%.......!....%.........!..%...........%...........%......]
60     ///                     beg            end
61     /// [ 1st window][ 2nd window][...
62     ///
63     /// With linear index, we can take the second window, find out what is 
64     /// the minimum virtual offset among alignments overlapping this window,
65     /// and skip all chunks which end position is less or equal to this offset:
66     ///
67     /// [........@...........!..............!.................................]
68     ///   .  ..min. offset   beg           end
69     ///   [  ).        .                              <- this chunk is skipped
70     ///       [        )                              <- this one is not
71     ///
72     VirtualOffset[] ioffsets; 
73 
74     /// Get (approximate) virtual offset of the first alignment overlapping $(D position)
75     /// 
76     /// Returned virtual offset is less or equal to real offset.
77     VirtualOffset getMinimumOffset(int position) {
78         int pos = max(0, position);
79         int _i = min(pos / BAI_LINEAR_INDEX_WINDOW_SIZE, cast(int)ioffsets.length - 1);
80         auto min_offset = (_i == -1) ? VirtualOffset(0) : ioffsets[_i];
81         return min_offset;
82     }
83 }
84 
85 struct BaiFile {
86     Index[] indices;
87 
88     /// Initialize from stream which contains BAI data
89     this(ref Stream stream) {
90         _stream = stream;
91         parse();
92     }
93 
94     /// Open BAI file given either filename of BAM file or that of BAI file.
95     this(string filename) {
96         Stream fstream;
97 
98         if (!endsWith(filename, ".bai")) {
99             /// Unfortunately, std.path.addExt is going to be deprecated
100 
101             auto first_filename = filename ~ ".bai";
102             auto second_filename = to!string(retro(find(retro(filename), '.'))) ~ "bai";
103 
104             if (std.file.exists(first_filename)) {
105                 fstream = new BufferedFile(absolutePath(first_filename));
106             } else {
107                 if (std.file.exists(second_filename)) {
108                     fstream = new BufferedFile(absolutePath(second_filename));
109                 } else {
110                     throw new Exception("searched for " ~ first_filename ~ " or " ~
111                                         second_filename ~ ", found neither");
112                 }
113             }
114         } else {
115             fstream = new BufferedFile(filename);
116         }
117 
118         Stream estream = new EndianStream(fstream, Endian.littleEndian);
119         this(estream);
120     }
121 
122 private:
123     Stream _stream;
124 
125     /// according to section 4.2 of SAM/BAM specification
126     void parse() {
127         auto magic = _stream.readString(4);
128         enforce(magic == "BAI\1", "Invalid file format: expected BAI\\1");
129 
130         int n_ref;
131         _stream.read(n_ref);
132         indices = uninitializedArray!(Index[])(n_ref);
133 
134         foreach (i; 0 .. n_ref) {
135             int n_bin = void;
136             _stream.read(n_bin);
137             indices[i].bins = uninitializedArray!(Bin[])(n_bin);
138             
139             foreach (j; 0 .. n_bin) {
140                 uint id = void;
141                 _stream.read(id);
142                 auto bin = Bin(id);
143 
144                 int n_chunk = void;
145                 _stream.read(n_chunk);
146                 bin.chunks = uninitializedArray!(Chunk[])(n_chunk);
147                 
148                 foreach (k; 0 .. n_chunk) {
149                     ulong tmp = void;
150                     _stream.read(tmp);
151                     bin.chunks[k].beg = VirtualOffset(tmp);
152                     _stream.read(tmp);
153                     bin.chunks[k].end = VirtualOffset(tmp);
154                 }
155                 
156                 indices[i].bins[j] = bin;
157             }
158 
159             int n_intv = void;
160             _stream.read(n_intv);
161             indices[i].ioffsets = uninitializedArray!(VirtualOffset[])(n_intv);
162 
163             foreach (j; 0 .. n_intv) {
164                 ulong tmp = void;
165                 _stream.read(tmp);
166                 indices[i].ioffsets[j] = VirtualOffset(tmp);
167             }
168         }
169     }
170 }